Composite
Trg-EnvZ

Part:BBa_K1731000:Design

Designed by: Alejandro Rodriguez-Gama, Lizbeth Airais Bolaños Castro   Group: iGEM15_UNAM-CU   (2015-09-14)

Constitutive Trg-EnvZ Chimera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Design Notes

Some restriction sites were removed without altering the open reading frame.


Source

Synthetic and codon optimized for E. coli expression

References